Scientist, Cell Engineer

3 weeks ago

Berkeley, United States Theery Full time

The position

Our client is looking for an extremely smart and hard working Cell Engineer with expert-level knowledge in various cell engineering methods, including experience with iPSCs and differentiated cells. The ideal candidate will have a deep understanding of advanced techniques such as:

  • Epigenetic targeting,
  • Global editing, CRISPRa, CRISPRi,
  • RNA-based reprogramming and will generally be well-versed in thinking about complex, cutting-edge methods to target cell differentiation using cell engineering tools.


As a critical member of their team, you will lead projects designing vectors for gene expression or silencing in stem cells to create specific cell types. You will work with their computational team to analyze single-cell RNA-seq data to target genes effectively and collaborate with our organoid team to develop organoid models using reprogrammed cells.


What they're looking for

It is important for them that you are aligned with their mission. You should have a strong solid scientific background with a reliable track record in designing and executing experiments independently.

  • Must have a PhD
  • You should have experience in transfecting (using viral or non-viral vectors) different cell types and ideally have run larger gene editing and/or transgene expression screens before.
  • You should have experience with different assays that could be used as a functional readout such as flow cytometry, immunofluorescence, and RNAseq.
  • Having experience with computational tools to analyze data is a big plus too.


Their Research

To accomplish their goal, they must derive human germ cells from iPSCs (human germ cell-like cells, hPGCLCs) that resemble both the transcriptional and epigenetic profile of in vivo germ cells (PGCs) that are able to enter meiosis and develop to viable human eggs. They create ovarian organoids from primary cells and stem cell derived cells to help the eggs develop properly.

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