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Research Associate III, Antibody Discovery

4 months ago

Bothell, United States NuWest Group Full time

Research Associate III, Antibody Discovery & Engineering


Pay Rate: $40-45/hr


Position Summary:

We are seeking a collaborative and independent Research Associate III (RA III) to participate in development of antibody-based oncology therapeutics. The RA III will report to the Molecular and Cellular Technologies team lead within Antibody Discovery. Their primary responsibilities will include engineering cell lines and performing flow-based assays to support antibody campaigns. The successful candidate will work well in a highly collaborative team environment and possess expertise in flow cytometry, mammalian cell culture, and cell engineering.


Responsibilities:

• Design, execute, troubleshoot, optimize, and interpret experiments

• Order constructs for overexpression and knockout cell line generation

• Engineer cell lines using transfection, nucleofection, CRISPR/Cas9, lentiviral transduction, and other methodologies

• Evaluate target expression on engineered and endogenous cell lines using flow cytometry and molecular characterization

• Perform routine cell culture maintenance and banking, working with both primary and cancer cells

• Optimize and perform flow cytometry assays to screen and characterize antibodies

• Maintain laboratory spaces, equipment, and supplies

• Keep an up-to-date and accurate electronic laboratory notebook

• Present experimental findings and contribute to scientific discussion at group and cross-functional team meetings

• Proactively seek guidance and build knowledge relevant to the role


Required Qualifications:

• Bachelor’s degree + 4 years laboratory research experience or Master’s degree + 2 years laboratory research experience in biological sciences or related subject

• Proficiency in culturing/maintaining multiple cell lines under BSL2 conditions

• Knowledge of cell engineering platforms, including transient transfection, generation of stable cell lines, and CRISPR

• Experience in multi-color flow cytometry

• Demonstrated ability to work independently and collaboratively, according to project needs

• Strong written and verbal communication skills, including ability to maintain a good working laboratory notebook


Preferred Qualifications

• Experience with FACS and single-cell cloning

• Basic molecular biology skills, including vector design, cloning, and PCR

• Knowledge of antibodies or antibody drug conjugates (ADCs)