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Associate Scientist
4 months ago
Job Summary
The Associate Scientist will perform genome mining of microbial genetic sequences, aiming to identify promising biosynthetic gene clusters for production of bioactive natural products, such as antibiotics and immunomodulatory agents. Following the identification of the gene clusters, the corresponding compounds will be produced, and the biosynthetic logic and associated enzymology will also be studied. In addition, the biological and/or physiological activities of the compounds discovered will be explored. Required Education and Experience The candidate must have a degree in chemistry, biochemistry or related field with at least three years of postdoctoral research experience by the start date of employment, plus expertise in natural products metabolomics, biosynthesis, and biological evaluation, as well as strong communication and writing skills in English. Preferred Qualifications Knowledge/Skills/Abilities Expertise in natural products metabolomics, biosynthesis and biological evaluation. Job Duties Job Duty Performs microbial genome mining, biosynthetic gene cluster cloning/capture and expression. Exploring microbial genomes to find genes or biosynthetic gene clusters (BGCs) that have a particular function, often related to the biosynthesis of natural products like antibiotics or other metabolites, using bioinformatic tools. After identification, the candidate will clone these genes or BGCs, typically using techniques like PCR amplification, TAR cloning, and vector-based transformation or conjugation, into more manageable host organisms, such as E. coli or Streptomyces to produce target compounds. The expression condition will be optimized as necessary to efficiently harness the novel compounds they produce, paving the way for potential applications in medicine. Essential Function Yes Percentage of Time 20 Job Duty Performs biosynthetic pathway and enzymology study. Investigation of biosynthetic pathways sequences of enzymatic reactions that lead to the synthesis of complex molecules from simpler precursors. The candidate will express and purify enzymes involved in the biosynthetic pathway, identify intermediates, determine the kinetic parameters of enzymes, further illuminate the enzymes specificity and mechanism. Essential Function Yes Percentage of Time 30 Job Duty Performs biological/physiological study of the compounds discovered. Facilitate a multifaceted exploration into the interactions, impacts, and implications of novel compounds. The candidate will conduct the in vitro assessment of the compounds, often utilizing cell lines to ascertain biological activities, such as potential cytotoxicity, cell proliferation, or specific biomarker modulation. Colonization of mice with certain microbe may also be performed, followed by analysis of a variety of biomarkers and/or phenotypes. Essential Function Yes Percentage of Time 30 Job Duty Work with the PI to write manuscripts and reports on funded projects. The candidate will lead the writing of journal articles and intellectual property disclosures emerging from research activities. It is expected that the successful candidate will be experienced in the preparation of publication-quality research artwork and narrative descriptions of results and background. The candidate will work with and receive feedback from the PI to continue to develop their expertise in the field. Essential Function Yes Percentage of Time 10 Job Duty Lab management: oversee graduate and undergraduate students in their research projects.The candidate will work with the PI to mentor graduate and undergraduate students in maintaining a productive laboratory environment. Essential Function Yes Percentage of Time 10 Position Attributes Hazardous weather category Non-Essential Employees in Safety-Sensitive or Security-Sensitive positions will be subject to pre-employment and post-employment drug testing in accordance with University policy HR Drug and Alcohol Testing. No Posting Detail Information