UNIV - Research Specialist II - Pediatrics: DCRI
4 days ago
Job Description
Cancer research laboratory in the Darby Children’s Research Institute at Department of Pediatrics has a job opening for a research technician II position. The lab uses in vitro, ex vivo and in vivo models as well as bioinformatics tools to elucidate the intracellular drivers of pediatric brain tumor growth.
The selected candidate will provide intellectual and technical support for RNA sequencing, single-cell RNA sequencing and spatial transcriptomic analysis as well as to be responsible for the computational maintenance and logistical organization of a translational science laboratory. We seek a highly motivated individual with previous experience in omics and molecular biology. The candidate will also be responsible for working with faculty in the Department of Pediatrics to assist with their research needs related to artificial intelligence (AI).
Candidate should send CV and a list of three references directly to Dr. Jezabel R Blanco by email ().
Collaborate with wet-lab and computational teams to employ various omics approaches for testing and refining biological hypotheses. (Essential 20%)
Upstream processing of omics’ data (QC, normalization, exploratory data analysis) (Essential 20%)
Downstream processing of bulk, single-cell and/or spatial transcriptomics data cell type annotation, differential gene expression analysis, gene set enrichment analysis, visualization, etc.) Use statistical and machine learning techniques to find relationships in complex biological data (Essential 20%)
Responsible for keeping accurate and documentation of bioinformatic pipelines, etc. (Marginal 5%).
Participate and lead monthly meetings with the DCRI faculty and its members to educate researchers in the use of AI tools (Essential 15%)
Complete xenograft and allograft mouse tumor models, including implantation of tumor cells, compound dosing, and in vivo tumor growth assessment. Tissue and cell isolation from mice, followed by immunohistochemistry, microscopy and a variety of cellular and molecular biology procedures (Marginal 10%)
Maintain several cell cultures and primary cultures in vitro. Bacterial recombinant DNA work and lentiviral production for in vitro transfection and transduction experiments. Perform a number of cell and molecular biology procedures, such as cell viability, IF, FACs analyses, immunoblotting, PCR, qPCR, reporter assays…) (Marginal 5%)
Participate in laboratory meetings, manuscript and grant preparation as deemed appropriate by the PI (Marginal 5%)
Minimum education: Bachelor’s degree in Biology, Chemistry, Molecular Biology or other life science required.
Preferred Knowledge, Skills and Abilities: Strong organizational, record keeping, interpersonal and communication skills required. Should be familiar with high-performance computing systems, shell scripting in a Linux environment, and have facility with R and/or Python including relevant bioinformatic packages Seurat, Deseq2, etc.). Prior experience with omics analysis and machine learning techniques is desirable. Must be able to perform complex calculations, to follow complicated experimental procedures and methods and develop independence with tasks and experiments. Some weekend, holidays and/or evenings may be required.
Additional Job Description
Minimum Requirements: A bachelor's degree in chemistry, biology, or other natural, life or health care science and one year work experience directly related to the assigned research area. Physical Requirements: (Note: The following descriptions are applicable to this section: Continuous - 6-8 hours per shift; Frequent - 2-6 hours per shift; Infrequent - 0-2 hours per shift) Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32° F to 100° F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted. (Infrequent) Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted. (Infrequent) Ability to push/pull file boxes from one location to another at floor level. (Frequent) Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted. (Frequent) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to maintain 20/40 vision, corrected, in one eye or with both eyes. (Continuous) Ability to match or discriminate between colors, normally. (Continuous) Ability to hear and recognize various alarms emitting from work area systems. (Continuous) Ability to hear and/or understand conversations. (Continuous) Ability to work in a darkroom for processing film, etc. (Continuous) Ability to be qualified physically for respirator use, initially and annually. (Continuous) Additional New Requirements: Ability to work rotating shifts as required. Ability to work overtime as required. Ability to learn and use new processes, tools and equipment as required.If you like working with energetic enthusiastic individuals, you will enjoy your career with us
The Medical University of South Carolina is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications, merit and business need.
Medical University of South Carolina participates in the federal E-Verify program to confirm the identity and employment authorization of all newly hired employees. For further information about the E-Verify program
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