Systems Validation Scientist 3

4 weeks ago

San Jose, United States MillenniumSoft Full time

Roles & Responsibilities

Must haves on the resume:
1. Best practice experience doing antibody methods for antigen labeling (, flow cytometry, western blots, ELISA, ELISPOT)
2. Experience working in a lab, with basic lab skills and knowledge (pipetting, dilutions, titrations, etc.)
3. Ability to purify leukocytes from mammalian-derived samples (, venous blood) via different density-gradient centrifugation methods and RBC lysis protocols.
4. Ability to stain cells in tubes and plates for intracellular and extracellular antigens.

Responsibilities:
The Systems Validation Scientist II will play a critical role in preparing samples for flow cytometric studies.

Responsible for:
• Maintaining the health, expansion, and inventory of cell lines
• Performing cryopreservation and thawing of mammalian cells
• Purification of leukocytes from mammalian-derived samples (, venous blood) via different density-gradient centrifugation methods and RBC lysis protocols.
• Staining of cells in tubes and plates for intracellular and extracellular antigens.
• Fixation and permeabilization of cells, treatment of cells in culture with various agents, maintaining documents/lot numbers of antibodies and reagents.
• Counting cells via hemocytometer and/or proprietary cell counters.
• Preparing buffers, media, and other reagents.
• Maintaining inventory of critical laboratory supplies (consumables and reagents) in the lab
• Attend meetings, as needed.

Experience Required

• At least 1-2 years of experience working in a Biosafety Level 2 laboratory environment, performing cell culture with multiple different mammalian cell lines.
• Best practice experience doing antibody methods for antigen labeling (, flow cytometry, western blots, ELISA, ELISPOT)
• Experience working with primary cells derived from various mammalian species.

Skills & Certifications

• Proficient with phase-contrast and brightfield microscopy.
• Excellent aseptic technique.
• Ability to troubleshoot common technical problems associated with cell sample preparation for flow cytometric studies.
• Ability to calculate basic laboratory calculations.
• Excellent attention to detail, can work quickly and accurately, organizing tasks and delivering quality with efficiency.
• Ability to read and understand Standard Operating Procedures
• Ability to interact with researchers for delivery of cells.
• Ability to maintain a clean work area.
• Knowledge of GLP/GDP guidelines.
• Experience working in a lab, with basic lab skills and knowledge (pipetting, dilutions, titrations, etc.)
• Excellent written and oral communication skills.

Eligibilities & qualifications

Qualifications:
The successful candidate will possess:
• A minimum of a bachelor’s degree in Microbiology, Biology, Biochemistry, Immunology, or a related field.

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