Sr. Scientist I

Found in: Resume Library US A2 - 1 week ago


North Chicago Illinois, United States AbbVie Full time
Job Description

Key Responsibilities

 

Design, execute, & interpret critical experiments to answer scientific questions.

Generate & test hypothesis by designing, performing, & interpreting laboratory-based experiments.

Read literature critically & analyze genomic data to support these activities.

Develop in vitro & in vivo screening assays in collaboration with therapeutic area scientists by generating cell lines, developing lentiviral & RNP delivery methods, & integrating & optimizing screening protocols.

Responsible for transfection, lentiviral transduction, flow cytometry, cell sorting, next generation sequencing, & performing CRISPR screens in neuronal & glial cell types.

Perform pooled & arrayed CRISPR screens with FACS-based molecular & functional assays on immortalized cell lines, primary cells, & iPSC-derived cells.

Work closely with NGS & bioinformatics scientists to process genomic samples, analyze screening results, & generate screening hits for follow-up studies.

Investigate novel screening hits in the context of disease biology by generating & testing hypothesis using a variety of biochemical, molecular, cellular, & genomic assays.

Utilize molecular analytic methods including DNA/RNA isolation, PCR/qPCR, molecular cloning, genotyping, &/or western blot.

Map candidate genes to known disease genetic networks through experimental & bioinformatic approaches.

Learn, understand, & master new experimental techniques, & act as a resource or mentor for others. Perform RNA-sequencing, RNAi (shRNA/siRNA), & CRISPR gene editing.

Collaborate and communicate with cross-functional scientists on experimental designs and results and scientific goals.

Report research progress to management on a regular basis, provide project updates to cross-functional project teams, and provide scientific presentations to department and other internal scientific forums.

Seek and advance opportunities to present research results at external scientific conferences & publish high-impact articles in peer-reviewed journals.

 


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