Lister Summer Studentship on Pathogen Interactions in Cystic Fibrosis

Aims and Objectives: Cystic fibrosis (CF) is a life-limiting genetic disorder which is often characterised by recurrent respiratory infections that significantly impact patient outcomes. These infections are polymicrobial and interactions between pathogens can contribute to worsening patient outcomes. The canonical CF pathogens include Pseudomonas aeruginosa, Staphylococcus aureus, Burkholderia cepacia complex (BCC) and non-tuberculosis mycobacterium (NTM) species, such as Mycobacterium abcessus. While much attention has been given to the individual impact of these pathogens, little is known about how they interact during co-infection. Emerging evidence suggests that these pathogens could either promote or inhibit the growth and virulence of co-infecting pathogens, potentially altering the course of infection and treatment response. Understanding these interactions could provide critical insights for developing more effective therapeutic strategies that target multiple pathogens simultaneously.

This study aims to investigate the interactions between the CF pathogens M. abcessus and B. cepacia as well as P. aeruginosa and S. aureus within a hydrogel biofilm model.

Benefits for Student: This project offers the student an opportunity to develop valuable microbiological research skills, many of which are not routinely covered at the undergraduate level.

These include:

Advanced microbial culture techniques, including pathogen co-culture and hydrogel models. Quantification of bacterial competition using CFU enumeration and inhibition assays. Molecular microbiology techniques, such as RNA extraction and qRT-PCR

In addition to technical skills, this project will provide the student with experience in independent research, enhancing problem-solving abilities, critical thinking, and data interpretation. The findings will contribute to a poster presentation and may be integrated into a future publication, offering the student valuable networking and academic development opportunities.

The University will actively foster a culture of inclusion and diversity and will seek to achieve true equality of opportunity for all members of its community.

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