Research Laboratory Technician

1 day ago


New York, New York, United States Columbia University Full time
Job Summary

Columbia University is seeking a highly skilled Technician B to provide bench and data analysis support for cell biology, molecular biology, immunology, and functional genomic assays. The successful candidate will assist the lab team with experiments and general research activities.

Key Responsibilities
  • Ensure compliance with study protocols, including IACUC and IRB, and laboratory safety.
  • Perform routine cellular and molecular assays, such as transformation, miniprep, maxiprep, RNA, DNA, and protein preparation, cDNA synthesis, qRT-PCR, transfection, packaging of viral vectors, ELISA, and Western Blotting.
  • Maintain primary mouse and human cell culture and human induced pluripotent stem cell culture and differentiation.
  • Utilize microscopic techniques and FACS for characterization/functional assays of cell lines and primary cells.
  • Perform histological sectioning, staining, imaging, and quantitative data analysis.
  • Assist with other lab team efforts, including breeding, genotyping, and preparation of animal models for experiments and surgeries.
  • Assist with sample preparation of RNA-seq, single cell RNA-seq, CRIPSR screening, high content imaging, and other functional genomic assays.
  • Interpret and present data, and participate in manuscript preparation.
  • Order and maintain laboratory supplies, chemicals, and equipment.
Requirements
  • Bachelor's degree in biology, chemistry, biochemistry, molecular biology, biostatistics, or other biomedical research-related fields or equivalent in education, training, and experience.
  • Experience in a research laboratory.
  • Familiarity with cell biology, molecular biology, immunology, and functional genomic techniques, and animal models.
  • Ability to develop skills in experimental design, data analysis, visualization, and interpretation.
Additional Requirements
  • Good organizational and communication skills.
  • Ability to work well as part of a team, as well as independently.
  • Must successfully complete systems training requirements.
  • Participation in the medical surveillance program.


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