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Systems Validation Scientist 3

2 months ago

San Jose, California, United States MillenniumSoft Full time

Job Summary

MillenniumSoft is seeking a highly skilled Systems Validation Scientist 3 to join our team. As a key member of our laboratory operations, you will play a critical role in preparing samples for flow cytometric studies.

Key Responsibilities

  • Maintain the health, expansion, and inventory of cell lines
  • Perform cryopreservation and thawing of mammalian cells
  • Purify leukocytes from mammalian-derived samples via different density-gradient centrifugation methods and RBC lysis protocols
  • Stain cells in tubes and plates for intracellular and extracellular antigens
  • Fixation and permeabilization of cells, treatment of cells in culture with various agents, maintaining documents/lot numbers of antibodies and reagents
  • Counting cells via hemocytometer and/or proprietary cell counters
  • Prepare buffers, media, and other reagents
  • Maintain inventory of critical laboratory supplies (consumables and reagents) in the lab
  • Attend meetings, as needed

Requirements

  • At least 1-2 years of experience working in a Biosafety Level 2 laboratory environment, performing cell culture with multiple different mammalian cell lines
  • Best practice experience doing antibody methods for antigen labeling (flow cytometry, western blots, ELISA, ELISPOT)
  • Experience working with primary cells derived from various mammalian species

Preferred Qualifications

  • Proficient with phase-contrast and brightfield microscopy
  • Excellent aseptic technique
  • Ability to troubleshoot common technical problems associated with cell sample preparation for flow cytometric studies
  • Ability to calculate basic laboratory calculations
  • Excellent attention to detail, can work quickly and accurately, organizing tasks and delivering quality with efficiency
  • Ability to read and understand Standard Operating Procedures
  • Ability to interact with researchers for delivery of cells
  • Ability to maintain a clean work area
  • Knowledge of GLP/GDP guidelines
  • Experience working in a lab, with basic lab skills and knowledge (pipetting, dilutions, titrations, etc.)
  • Excellent written and oral communication skills

Education

  • A minimum of a bachelor's degree in Microbiology, Biology, Biochemistry, Immunology, or a related field