Immunology Research Associate
1 day ago
The Alaka`ina Foundation Family of Companies is seeking an Immunology Research Associate to support our government customer located in Ft. Detrick, Maryland. As a key member of our team, you will be responsible for conducting immunological assays, including high-parameter flow cytometry, ELISpot, and ELISAs. You will also be responsible for implementing study/project request processes, planning and executing scientific activities, and generating high-quality interim and final study reports.
Responsibilities:
- Perform in-life and post-life immunological assays, including high-parameter flow cytometry and analysis, ELISpot, and ELISAs.
- Implement study/project request process utilizing standardized study proposal templates.
- Plan and execute scientific activities, including drafting and preparing paperwork, reagent preparation, and preparation of flow cytometry master mixes and reagents.
- Generate high-quality interim and final study reports, and develop them for timely publication in peer-reviewed journals.
- Perform regular maintenance and laboratory operations within BSL-2 and BSL-4 laboratories, including equipment quality controls, media preparations, routine cleaning, and hazardous waste management.
- Actively participate in practices and processes to enhance data quality and integrity, including periodic review of study execution to identify gaps and refine procedures.
- Participate in training and cross-training programs to assure continuity of operations and scientific core activities.
- Develop and revise Standard Operating Procedures (SOPs) and methods for laboratory operations.
- Comply with NIH Division of Occupational Health and Safety requirements for satisfactory completion of the Biosurety program and Select Agent program.
- Other duties as assigned.
Requirements:
- Bachelor of Science or higher degree in Microbiology, Virology, Immunology, or similar field.
- Two or more years of experience in conducting immunological assays, including immunophenotyping/flow cytometry, FACS, Luminex cytokine profiling, and analysis, magnetic bead enrichments for population analysis, ELISpot, and ELISAs.
- Ability to work in a BSL-4 setting with select agents.
- Proficiency in Word, Excel, PowerPoint, and GraphPad Prism.
- Experience with models of viral infectious diseases.
- Ability to work both independently and within small and large team settings.
- Ability to organize, analyze, summarize, and perform quality checks of data.
- Ability to be flexible regarding occasional schedule changes due to in-life study support work.
- Must be willing to participate in periodic drug screening.
- Must have the ability to obtain/maintain certification in Biological Personnel Reliability Program and CDC Select Agent Program approval.
Desired Skills and Experience:
- Desire to learn and implement a diverse set of skills and techniques related to immunological assays.
- Two or more years of recent experience conducting laboratory assessments in BSL-3 or BSL-4 laboratory settings.
- Proficiency in Cytek SpectroFlo and flow cytometry data analysis, including FlowJo, FCS Express, OMIQ, or FlowSOM.
- Experience with isolation of primary cells from tissues, cell culture, and sterile technique.
- Experience in virological assays, including PRNT and TCID50.
- Background in assay development and optimization.
- Basic understanding of laboratory information management systems (LIMS).
- Familiarity with optical imaging modalities, including in vivo fluorescence and bioluminescence imaging.
- Experience with super-resolution microscopy modalities, including dSTORM and PALM.
Clearance and Citizenship Requirements:
- Must be a U.S. Citizen.
- Ability to obtain/maintain DoJ Security Risk Assessment (SRA) approval.
- Biosurety Acknowledgement.
- Active Secret T3 Clearance.
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