Immunology Research Associate

1 day ago


Frederick, Maryland, United States Alaka`ina Foundation Family of Companies Full time
Job Title: Immunology Research Associate - Laboratory Operations

The Alaka`ina Foundation Family of Companies is seeking an Immunology Research Associate to support our government customer located in Ft. Detrick, Maryland. As a key member of our team, you will be responsible for conducting immunological assays, including high-parameter flow cytometry, ELISpot, and ELISAs. You will also be responsible for implementing study/project request processes, planning and executing scientific activities, and generating high-quality interim and final study reports.

Responsibilities:

  • Perform in-life and post-life immunological assays, including high-parameter flow cytometry and analysis, ELISpot, and ELISAs.
  • Implement study/project request process utilizing standardized study proposal templates.
  • Plan and execute scientific activities, including drafting and preparing paperwork, reagent preparation, and preparation of flow cytometry master mixes and reagents.
  • Generate high-quality interim and final study reports, and develop them for timely publication in peer-reviewed journals.
  • Perform regular maintenance and laboratory operations within BSL-2 and BSL-4 laboratories, including equipment quality controls, media preparations, routine cleaning, and hazardous waste management.
  • Actively participate in practices and processes to enhance data quality and integrity, including periodic review of study execution to identify gaps and refine procedures.
  • Participate in training and cross-training programs to assure continuity of operations and scientific core activities.
  • Develop and revise Standard Operating Procedures (SOPs) and methods for laboratory operations.
  • Comply with NIH Division of Occupational Health and Safety requirements for satisfactory completion of the Biosurety program and Select Agent program.
  • Other duties as assigned.

Requirements:

  • Bachelor of Science or higher degree in Microbiology, Virology, Immunology, or similar field.
  • Two or more years of experience in conducting immunological assays, including immunophenotyping/flow cytometry, FACS, Luminex cytokine profiling, and analysis, magnetic bead enrichments for population analysis, ELISpot, and ELISAs.
  • Ability to work in a BSL-4 setting with select agents.
  • Proficiency in Word, Excel, PowerPoint, and GraphPad Prism.
  • Experience with models of viral infectious diseases.
  • Ability to work both independently and within small and large team settings.
  • Ability to organize, analyze, summarize, and perform quality checks of data.
  • Ability to be flexible regarding occasional schedule changes due to in-life study support work.
  • Must be willing to participate in periodic drug screening.
  • Must have the ability to obtain/maintain certification in Biological Personnel Reliability Program and CDC Select Agent Program approval.

Desired Skills and Experience:

  • Desire to learn and implement a diverse set of skills and techniques related to immunological assays.
  • Two or more years of recent experience conducting laboratory assessments in BSL-3 or BSL-4 laboratory settings.
  • Proficiency in Cytek SpectroFlo and flow cytometry data analysis, including FlowJo, FCS Express, OMIQ, or FlowSOM.
  • Experience with isolation of primary cells from tissues, cell culture, and sterile technique.
  • Experience in virological assays, including PRNT and TCID50.
  • Background in assay development and optimization.
  • Basic understanding of laboratory information management systems (LIMS).
  • Familiarity with optical imaging modalities, including in vivo fluorescence and bioluminescence imaging.
  • Experience with super-resolution microscopy modalities, including dSTORM and PALM.

Clearance and Citizenship Requirements:

  • Must be a U.S. Citizen.
  • Ability to obtain/maintain DoJ Security Risk Assessment (SRA) approval.
  • Biosurety Acknowledgement.
  • Active Secret T3 Clearance.


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