Senior Cell Biologist

2 weeks ago

Irvine, California, United States Spectraforce Technologies Full time
Job Title: Associate Scientist

Job Summary:

The Associate Scientist will work independently and collaboratively as part of a team to contribute expertise to projects focused on designing, developing, and optimizing novel in vitro complex cell models. The successful candidate will have a strong background in molecular biology, cell and organ culture techniques, and stem cell culture and induced cell differentiation.

Key Responsibilities:

  • Design and optimize protocols for the efficient and reproducible differentiation of iPSCs into specific cell types.
  • Characterize and validate the differentiated cells using molecular and functional assays to ensure their fidelity and functionality.
  • Develop and optimize advanced cell models, including co-culture systems, organoids, or tissue-on-a-chip platforms, using iPSC-derived cells to mimic physiological or disease conditions.
  • Optimize cell culture protocols, media formulations, and growth conditions to ensure the viability, functionality, and reproducibility of the developed cell models.
  • Collaborate with cross-functional teams to integrate the developed cell models into larger research projects, including supporting in vivo model development and in vivo studies.
  • Plan experimental approaches with supervisory guidance and discuss, recommend, and modify experimental techniques as needed.
  • Document and interpret results to reach conclusions and discuss with supervisor to decide on subsequent experiments.
  • Prepare technical reports, presentations, and documentation of work performed.

Requirements:

  • Proven experience in iPSC maintenance, reprogramming, and differentiation techniques using various differentiation protocols.
  • Proficiency in advanced cell culture techniques, including iPSC culture and differentiation, primary cell isolation, immortalized cell lines, three-dimensional (3D) cell culture systems, co-culture systems, and organoid culture systems.
  • Experience in characterizing and validating differentiated cells using functional assays and molecular markers.
  • Proven experience in molecular biology techniques for analysis of in vitro cell-based assays and ex vivo tissues and/or fluid (RNA isolation, qPCR, western blot, ELISA, Luminex, F/ISH, IHC, flow cytometry, etc.).
  • Proficient in data analysis software suites (e.g., GraphPad PRISM, Excel, R, etc.).
  • Excellent problem-solving skills and the ability to troubleshoot technical issues independently.
  • Strong organizational skills and attention to detail to maintain accurate records and documentation.

Education: Bachelor's Degree or equivalent education, or Master's Degree or equivalent education with years of experience.

Theoretical and practical knowledge to carry out the job functions.

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