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Assistant Core Technician
3 weeks ago
Provides support to perform the required standard operating procedures of the Core Research Facility. This includes the execution of research activities associated with established protocols. Works with Core Technician or Sr Core Technician in utilizing the facility's specialized equipment and services.
**Specific Responsibilities**:
Administration
- Performs intake functions for the laboratory such as logging of samples and service requests, and the dissemination of basic information to users (i.e.,
- Maintains established laboratory inventories.
- Places orders.
- Records work accurately and completely.
Collaboration
- Interacts primarily with other Core Staff.
Compliance
- Follows University safety protocols, relative to the use of combustibles, radioactivity, toxic material, and/or carcinogenic.
- Maintains sterile conditions as required..
- Prepares reagents.
Scientific
- Conducts experiments, assays, and records results.
- Reads and follows experimental protocols.
- Calculates, compiles, and communicates results to the Core Scientist.
Technical
- Prepares and operates equipment for experiments, assays, measurements,
- Supports the execution and facilitation of specialized services.
- Assists in performing minor equipment repairs, according to manufacturer
- Cleans and performs general maintenance on equipment, according to
- Abides by all University, sponsor and federal rules and regulations.
- Using an entry level of skill, completes the following activities
Molecular Biology:
- DNA, RNA, Protein isolation, purification and expression
- Southern, Northern, Western blotting
- PCR, RT-PCR
- Plasmid preparation
- Cloning, mapping and sequencing of DNA
- Recombinant virus production and purification
Cellular Biology:
- Cell culture, cell isolation
- Functional studies
- Transfection
- Immunofluorescence assays
- Image analysis and quantification
Chemistry:
- Analyze organic chemistry
- Column chromatography
- Chromatography
- Enzymatic digestions
- HPLC
- NMR
- Mass spectrometry
- Protein extractions
- Solid phase extraction
Histology:
- Tissue fixation/processing: embedding, preparation of
- Immunohistochemistry
Microscopy Immunology:
- Flow cytometry
- ELISA, Elispot assay
- Cytokine assay
- Antibody production and purification
Animal:
- Husbandry
- Breeding and screening transgenic mice
- Dissections
- Blood collection
- Surgical procedures
- Database management of colony
Equipment Used:
- Analytical balance
- Autoclave
- Blood gas analyzers
- Centrifuge
- Electrophoresis unit
- pH meter
- UV/Vis Spectrophotometer
- Scintillation counter
- pH meter, plate reader
Other (please specify):
- DNA/RNA quality and quantity check
- Reporting sample QC results to users
- Prepare DNA/RNA samples for conduct
- of next-gen sequencing
Miscellaneous
- Completes other duties as assigned.
**Minimum Qualifications: (Education, experience, and any other certifications or clearances)**
- HS diploma or equivalent and some basic science courses is required.
- Target hiring range for this position will be between $17.88 to $25.15per hour. Offered salary will be determined by the applicant’s education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data_.
**_ Benefits:_**:
**_ Work-Life and Wellness:_**:
- Northwestern offers comprehensive programs and services to help you and your family navigate life’s challenges and opportunities, and adopt and maintain healthy lifestyles._
**_ Professional Growth & Development:_**:
Northwestern strongly recommends COVID-19 vaccinations and boosters for people who can obtain them as a critical tool for minimizing severe illness. More information can be found on the COVID-19 and Campus Updates webpage.
- The Northwestern campus sits on the traditional homelands of the people of the Council of Three Fires, the Ojibwe, Potawatomi, and Odawa as well as the Menominee, Miami and Ho-Chunk nations. We acknowledge and honor the original people of the land upon which Northwestern University stands, and the Native people who remain on this land today._
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