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Postdoctoral Fellow

3 months ago

Spring House, United States Johnson & Johnson Full time

Johnson & Johnson is currently seeking a Postdoctoral Fellow - Cell Therapy, Genomic Safety Discovery to join our Johnson & Johnson Innovative Medicine (J&J IM) Team located in Spring House, PA.

For more than 130 years, diversity, equity & inclusion (DEI) has been a part of our cultural fabric at Johnson & Johnson and woven into how we do business every day. Rooted in Our Credo, the values of DEI fuel our pursuit to create a healthier, more equitable world. Our diverse workforce and culture of belonging accelerate innovation to solve the world’s most pressing healthcare challenges.

We know that the success of our business - and our ability to deliver meaningful solutions - depends on how well we understand and meet the diverse needs of the communities we serve. Which is why we foster a culture of inclusion and belonging where all perspectives, abilities and experiences are valued and our people can reach their potential.

At Johnson & Johnson, we all belong.

The J&J IM Discovery, Product Development, & Supply (DPDS) Postdoctoral Fellowship Program helps early career scientists gain the training and experience needed to further their careers while addressing the needs of global healthcare. Postdocs work side-by-side with DPDS scientists on innovative research projects and have the opportunity to present and publish their work. They also learn the functional role of scientists in the pharmaceutical industry and receive valuable career advice from mentors while building a network of professional contacts and developing the skills needed to advance their careers.

The anticipated start date for this role is October 14th, 2024.

Key Responsibilities:

- Collaborate with various functions within Johnson & Johnson such as cell therapy, protein engineering, and genetic safety.
- Culturing and maintaining induced pluripotent stem cells.
- Perform cloning, expressions, purification of proteins and assess functional characteristics.
- Use molecular biology techniques such as DNA/RNA isolation and next generation sequencing to assess the function of proteins.
- Partner with bioinformatics team to perform analysis and interpretation of the results.
- Adhere to good laboratory practices.

**Qualifications**:
**Education**:

- Degree must be completed in the last 3 years, or by October 2024 for current students

Required:

- Strong expertise designing, testing, and optimizing CRISPR/Cas knock-in/ knockout gene delivery systems.
- Hands on experience in cloning and expression vectors.
- Outstanding capacity to learn quickly and rigorously draw accurate conclusions from data.
- Self-motivated, with the ability to multitask, independently advance research programs and to collaborate well in a multi-disciplinary environment.
- Excellent communication skills (both oral and written)
- Ability to stay organized, plan for complicated long-term projects, and maintain detailed records.
- Track record of first author publications in scientific journals.

Preferred:

- Experience in culturing mammalian cells or immortalized cell lines or induced pluripotent stem cells (iPSC).
- Hands-on experience in gene editing primary cells or iPSCs.
- Strong understanding of stitching various proteins and epitopes, including protein expression and purification.
- Deep understanding of utilizing next generation sequencing data.
- Understanding the allogeneic cell therapy end-to-end process and currently used orthogonal assays for detecting off-targets.
- Knowledge about current US FDA and EMA guidelines for CAR-T and gene editing is a plus.
- Skills: DNA/ RNA/ Protein isolation, cell culture, flow cytometry, ELISA, western blot, qPCR, gene knockout, microscopy, primer design, protein purification and functional assay.

The salary for this position is $95,000/year, this position is eligible for a sign-on bonus. This is a two year duration based role that has eligibility for severance.

The Company maintains highly competitive, performance-based compensation programs. Under current guidelines, this position is eligible for an annual performance bonus in accordance with the terms of the applicable plan. The annual performance bonus is a cash bonus intended to provide an incentive to achieve annual targeted results by rewarding for individual and the corporation’s performance over a calendar/performance year. Bonuses are awarded at the Company’s discretion on an individual basis.

Employees and/or eligible dependents may be eligible to participate in the following Company sponsored employee benefit programs:

- Medical, dental, vision, life insurance, short
- and long-term disability, business accident insurance, and group legal insurance.
- Employees may be eligible to participate in the Company’s consolidated retirement plan (pension) and savings plan (401(k)).
- This position is eligible to participate in the Company’s long-term incentive program.
- Employees are eligible for the following time off benefits:

- Vacation - up to 120 hou