Biomedical Technician- Applied Genomics

3 weeks ago

Beverly Hills, United States CEDARS-SINAI Full time

**The Applied Genomics, Computation & Translational Core is looking for a Biomedical Technician to join the team**

The Cedars-Sinai Applied Genomics, Computation, and Translational Core (AGCT Core) is a fully equipped, state-of-the-art genomics facility offering data generation and interpretation for basic science and translational research in next-generation sequencing technologies, including single cell omics, spatial transcriptomics, and bulk cell omics. We specialize in single cell omics and spatial transcriptomics via the 10x Genomics, Parse Biosciences, Mission Bio, and NanoString platforms. Our R&D team offers sample preparation for single cell assays, such as nuclei isolation from frozen tissue or single cell isolation from fresh tissue dissociation. The AGCT Core also offers comprehensive services for standard bulk cell sequencing in genomics, transcriptomics, epigenomics, and metagenomics. To learn more please visit Applied Genomics, Computation & Translational Core | Cedars-Sinai.

**Are you ready to be a part of breakthrough research?**

The Genomics Core Biomedical Technician participates in wet
- and dry
- laboratory projects and may execute tasks requiring compliance with all Federal and State accrediting agency requirements and/or Good Laboratory Practices (GLP). Responsible for assigned wet lab work, lab maintenance, and writing and recording controlled documents. Performs all activities strictly according to SOPs, with accurate documentation, and may assist quality control team with required activities. Installs and maintains laboratory equipment to ensure proper functioning of devices. Assists in addressing complex issues and troubleshooting.

**Primary Duties and Responsibilities**:

- Performs a variety of project-specific process development tasks for multiple clients following standard operating procedures (SOPs).
- Maintains good documentation in laboratory notebooks, data record/transfer, SOPs, and batch records.
- Drafts standard operating procedures and maintains computer database.
- Verifies samples received, logs samples into systems, and may transport to appropriate environment within the facility for storage following SOPs.
- Performs lab maintenance duties, including glassware cleaning and sterilization.
- Maintains lab equipment and related records.
- Prepares cGMP production room, maintains material inventories, and places orders for equipment and supplies.
- Assists in the operation of specialized equipment and machinery.
- Ensures all activities comply with regulatory guidelines and safety standards, as appropriate.

**Department-Specific Responsibilities**:

- Perform manual library preparation for bulk cell, single cell, and spatial genomics.
- Understand and perform all aspects of NGS library sequencing, including library QC analysis, checking index overlap, determining sequencer loading concentration, determining sequencing run parameters, and determining reads needed.
- Consult with technical support on library preparation or sequencing run performance issues.
- Understand the chemistry of all NGS assays performed in the Core.
- Troubleshoot library preparation, sequencing, and equipment issues.
- Work closely with and guide RA I on performing and analyzing sample and library quantification and QC, and automated library preparation for service requests.
- Perform sample preparation for single cell assays.
- Perform and analyze NanoDrop quantification, Qubit quantification, Bioanalyzer QC, and TapeStation QC.
- Perform Illumina Sequencing by Synthesis sequencing, and understand and analyze sequencing run metrics.
- Perform DNA/RNA isolation from various sample types.
- Perform fold dilution, normalization, and conversion calculations.
- Liaise with researchers on quantification-, QC-, and sequencing-only requests, as needed.
- Use iLab as a laboratory information system for service requests and billing. Edit charges, as needed, to reflect the work performed as opposed to the work requested.
- Use LabArchives as an electronic laboratory notebook to record all information and data generated.
- Use Trello to track projects, record work performed, and plan work schedule.
- Update pertinent information to LabArchives and/or Trello on a daily basis,
- Understand how to choose the appropriate reagent kit to use based on sample quality and concentration.
- Properly handle and work with DNA and RNA samples.
- Understand the space layout of the lab and what work is performed in what space.
- Receive and store samples, and document receipt.
- Perform automated library preparation.
- Unpack orders and appropriately store reagents according to temperature requirements.
- Request reagents and consumables for order as needed.
- Understand next-generation sequencing (NGS).
- Report results and project status on a daily basis and at weekly lab meetings.
- Prepare PowerPoint presentations on NGS topics to present to team members.
- Maintain general lab cleanliness and organization, i

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