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Associate Scientist

2 months ago

Miami FL United States University of Miami Full time
Job DescriptionCurrent Employees:

If you are a current Staff, Faculty or Temporary employee at the University of Miami, please click here to log in to Workday to use the internal application process. To learn how to apply for a faculty or staff position using the Career worklet, please review this tip sheet.

Department Specific Functions

The Sylvester Comprehensive Cancer Center at the University of Miami Miller School of Medicine is looking for an Associate Scientist to join the new Sylvester Proteomic Shared Resource (PSR), to apply state of the art LC-MS based methods and techniques for quantitative proteomics.

As an Associate Scientist in the PSR, you will be responsible for the maintenance and operation of a modern high resolution orbitrap mass spectrometer as well as performing detailed biochemical sample processing techniques. In addition, you will be part of a small, agile, interdisciplinary team that seeks to invent innovative methods that can be used in the development of diagnostics aimed at getting the right treatment to right patients and understanding the fundamental underpinnings of cancer biology. The individual will:

  • Be the primary person responsible for the use of a new Orbitrap Ascend Tribrid LC-MS instrument for proteomic applications.
  • Manage inventory of lab consumables, including ordering, receiving, and storage of supply stocks.
  • Prepare protein samples from a wide range of specimen (e.g., cells, tissues, biofluids, and SDS Page gels)
  • Implement of both bottom-up & top-down proteomic approaches.
  • Be responsible for global proteome profiling by data-dependent (DDA) and data-independent (DIA) acquisition mass spectrometry and mass bar-codes. Detection of post-translational modifications (e.g., phosphorylation, acetylation).
  • Perform relative quantification of proteins by label-free and stable-isotope labelling strategies (e.g., SILAC, TMT).
  • Be responsible for structural proteomics (e.g., cross-linking, pull-down experiments).
  • Conduct single-cell proteomics analysis.
  • Perform intact protein analysis & MW determination.
  • Use proteomics software (e.g., Proteome Discoverer, MASCOT) for data analysis.
  • Be responsible for operation, maintenance, and troubleshooting of a Orbitrap Ascend Tribrid Mass Spectrometer and other laboratory equipment as needed.
  • Assess experimental results and troubleshoot methods and instruments to optimize research protocols.
  • Collaborate with technicians, graduate students, post-docs, PIs, the laboratory director & colleagues.

Education:

  • Ph.D. in Science or Engineering (Biochemistry, Bioengineering, Biology, Chemistry, or related field)

Experience:

Minimum 5 years of relevant experience. Five years of related experience with mass spectrometry instruments which includes 2 years of experience in proteomics mass spectrometry applications.

Knowledge, Skills and Attitudes:

  • Hands-on experience operating and/or maintaining LC-MS instruments and supporting equipment (including auxiliary systems such as chillers, controls, electronics, pumps, vacuum systems, etc.)
  • Strong interest in handling, processing, and analyzing high-throughput datasets.
  • Motivation to constantly develop skills and expertise.
  • Ability to work both independently and as a team member while engaging in the organization of the proteomics core.
  • Excellent analytical, communication, problem solving, and organizational skills.
  • excellent written and oral communication skills.
  • Established track record of publications and demonstrated ability to complete projects.
  • Capability to learn complex tasks with instruction and to use experience to perform a variety of new techniques.

Preference will be given.

  • Expertise in one or more of the following technical areas: quantitative proteomics, single cell proteomic analysis, global proteome profiling.
  • Previous hands-on operation of Thermo Tribrid Orbitrap LC-MS Systems (e.g., Fusion, Eclipse, Ascend).

#LI-YC1

The University of Miami offers competitive salaries and a comprehensive benefits package including medical, dental, tuition remission and more.

UHealth-University of Miami Health System, South Florida's only university-based health system, provides leading-edge patient care powered by the ground breaking research and medical education at the Miller School of Medicine. As an academic medical center, we are proud to serve South Florida, Latin America and the Caribbean. Our physicians represent more than 100 specialties and sub-specialties, and have more than one million patient encounters each year. Our tradition of excellence has earned worldwide recognition for outstanding teaching, research and patient care. We're the challenge you've been looking for.

Patient safety is a top priority. As a result, during the Influenza ("the flu") season (September through April), the University Of Miami Miller School Of Medicine requires all employees who provide ongoing services to patients, work in a location (all Hospitals and clinics) where patient care is provided, or work in patient care or clinical care areas, to have an annual influenza vaccination. Failure to meet this requirement will result in rescinding or termination of employment.

The University of Miami is an Equal Opportunity Employer - Females/Minorities/Protected Veterans/Individuals with Disabilities are encouraged to apply. Applicants and employees are protected from discrimination based on certain categories protected by Federal law. Click here for additional information.

Job Status:Full timeEmployee Type:StaffPay Grade:A11