Laboratory Technician III

4 weeks ago

Gainesville, United States University of Florida Full time

Classification Title:

Laboratory Technician III

Job Description:

This is a Lab Technician III position in a research lab developing immunotherapies for cancers. The Lab Technician III is expected to be able to 1) independently modify protocols to improve existing techniques or to incorporate new diagnostic procedures; 2) complete the execution of assigned experiments; 3) assist in maintaining a necessary rodent colony to achieve research objectives; 4) assist in performing rodent procedures as necessary to complete research objectives; 5) present researching findings to PI and at laboratory meetings; 6) maintain laboratory safety programs and train personnel in laboratory safety techniques; and 7) maintain detailed records of experiments. The Lab Technician III must be able to perform, mostly independently, the following essential functions:

* Tissue culture: Performing tissue culture/cell culture of primary cells or cell lines using aseptic techniques. Perform multiparameter flow cytometry and develop proficiency setting multicolor flow panels. Perform RNA and DNA isolation as well as in vitro transcription. Plasmid bacterial infection, plasmid transduction, and cloning are requirements for this position.
* Animal Studies: Participating in treating animals with pharmacological agents or immunotherapy through i.p., i.m., or i.v. injection. Plays a key role in regulatory management (IACUCs).
* Histological Work/Analysis: Collecting organs and other tissues and preparing for histopathology Following the preparation of slides, staining with standard histology stains such as H&E, as well as with immunohistochemistry and immunofluorescence will be done. Conduct image capture with fluorescent microscope, confocal microscope, multiphoton confocal microscope and analysis of tissue following intervention studies.
* Regulatory Compliance: Ensures the laboratory is compliant with governing rules and regulations. Coordinate all IACUC and IRB (exempt) submissions/renewals. Ensure all EH&S standards are met.
Train new laboratory members to ensure compliance standards are met.
* Training: Train and supervise graduate and undergraduate students performing diagnostic procedures related to immune therapy experiments.
* Any other duties, as needed, to fulfill the mission and to abide by the values of the College of Medicine. This will include performing tasks and experiments as directed by the Principal Investigator.

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