Staff Research Associate II
3 weeks ago
General Information
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Work Location: Los Angeles, USA
Work Schedule
Monday - Friday, 8:00am-5:00pm
Posted Date
08/09/2021
Salary Range: $28.04 - 45.11 Hourly
Employment Type
2 - Staff: Career
Duration
Indefinite
Job #
2684
Primary Duties and Responsibilities
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The Environmental Cardiology and Vascular Biology Laboratory is seeking to hire a Research Assistant to perform experiments in the areas of vascular and molecular biology, lipidology, pathology, and toxicology. Experiments include: histological analyses of aortic and liver sections for atherosclerotic lesions and hepatic steatosis; pulmonary macrophage transplantation studies; bone marrow transplantation studies; assessment of gene expression by quantitative PCR in lungs, liver and aortas of animals exposed to air pollutants and e-cigarettes. Primary duties:
* Conduct molecular biological procedures such as DNA and RNA extraction, gel electrophoresis, genotyping, western blots, real-time PCR, ELISAs, FPLC, ultracentrifugation
* Determine tissue and cellular oxidative stress through various techniques, including assessment of oxidized lipids by mass spectrometry. Perform tissue culture experiments and analyze histological samples
* Mouse colony management
* Perform bleeding, bronchoalveolar lavage, harvest of peritoneal macrophages, culture of bone marrow-derived macrophages, intratracheal administration of compounds, tissue and organ isolation
* Assist with preparation of manuscripts, grant submissions, and figures
Salary range: $28.04-$45.11 Hourly
Job Qualifications
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* Knowledge and proficiency in cellular and molecular biology techniques
* Ability to perform tissue culture experiments
* Ability to perform animal dissections
* Experience with mouse colony management
* Ability to perform technical procedures in a timely and accurate manner
* Knowledge and ability to operate basic laboratory equipment such as balances, pH meter, pipettes, light microscopes, centrifuges
* Ability to operate laboratory equipment such as FPLC/HPLC, ultracentrifuge
* Ability to follow written protocols and maintain records and logs accurately.
* Proficient in Microsoft Office Suite
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