Research Technician IV

4 weeks ago


Tucson, United States University of Arizona Full time

Perform highly technical experiments with minimal supervision including performing electroporation, transfection, and infection to introduce donor DNA into iPSC lines. Operate, upkeep, and troubleshoot sophisticated issues with various advanced scientific apparatuses, machinery, and tools, including FACS machine, electroporator, pH meter, Nanodrop, and Gel Imager, using specialized knowledge, skills, and independent judgment.Refine, adapt, or develop highly skilled procedures or techniques to fit specific experimental requirements. This includes reprograming iPSCs, CRISPR gene editing, cell culture, differentiation, and biobanking. Evaluate for process improvements to increase efficiency, optimize testing procedures, and reduce errors. Execute intricate procedures that necessitate precise handling and adjustments of equipment which includes maintaining sterile cultures and treating iPSC derived cells with compounds/growth factors.Collaborate with research team in the development of new strategies, techniques involving the differentiation of cells into various cell types. Coordinate with other labs on collaborative projects. Aid with independent development of new methods or approaches to develop iPSC models for investigators to study disease. Provide expert knowledge and support for team with intricate matters that require strong decision-making. Provide guidance, mentorship, and training to graduate and undergraduate students working the the team.Evaluate and maintain detailed records of research results, data acquisition, and data and statistical analysis of studies conducted. Data will be used for publications, future contract proposals, and/or provided to other PI's for use in their own research. Provide research support to the lab including stocking and ordering of supplies.



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