Research Associate I

3 weeks ago

Tampa, United States Moffitt Cancer Center Full time

At Moffitt Cancer Center, we strive to be the leader in understanding the complexity of cancer and applying these insights to contribute to the prevention and cure of cancer. Our diverse team of over 9,000 are dedicated to serving our patients and creating a workspace where every individual is recognized and appreciated. For this reason, Moffitt has been recognized on the 2023 Forbes list of America's Best Large Employers and America's Best Employers for Women, Computerworld magazine's list of 100 Best Places to Work in Information Technology, DiversityInc Top Hospitals & Health Systems and continually named one of the Tampa Bay Time's Top Workplace. Additionally, Moffitt is proud to have earned the prestigious Magnet designation in recognition of its nursing excellence. Moffitt is a National Cancer Institute-designated Comprehensive Cancer Center based in Florida, and the leading cancer hospital in both Florida and the Southeast. We are a top 10 nationally ranked cancer center by Newsweek and have been nationally ranked by U.S. News & World Report since 1999.

Working at Moffitt is both a career and a mission: to contribute to the prevention and cure of cancer. Join our committed team and help shape the future we envision.

Summary

Summary

The laboratory of Dr. Jiandong Chen at Moffitt Cancer Center Department of Molecular Oncology conducts basic research to understand the p53 tumor suppressor pathway and development of novel therapeutic strategies to target p53 for cancer therapy. The lab routinely performs mechanistic studies of protein expression, function, and regulation in response to novel drugs and stress treatments using cell culture and mouse models. The lab has extensive collaborations with chemists and structural biologists working in drug discovery, providing a rich environment for training and career development.

Position Highlights:

* Analysis of novel compounds from chemistry collaborators including inhibitors and PROTACs.
* Use mouse models to investigate efficacy of novel cancer drugs.
* Cell culture, CRISPR/Cas9 gene editing, creation of stable and inducible cell lines.
* Provides training in multiple techniques required by the projects.

The Ideal Candidate:

* Experience with cell culture and bacteria culture.
* Experience with Western blot, PCR, RT-PCR, FACS.
* Experience with recombinant DNA techniques.
* Experience with mouse xenograft models or genetically modified mice.

Responsibilities:

* Cell culture and drug treatment.
* Analysis of samples by Western blot, RT-PCR.
* Molecular cloning, plasmid purification.
* Recombinant protein expression in bacteria and purification.
* Perform transfection, luciferase reporter assays.
* Maintain mouse strains by PCR genotyping.
* Generation and treatment of tumor xenografts.
* Preparation of buffers and solutions.

Credentials and Qualifications:

* Bachelor's degree in biology or related field is required.
* Hands on research experience required.
* Proficient in calculating drug molar concentrations.

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