TECHNICAL RESEARCH ASSISTANT I BWH NEUROLOGY
3 weeks ago
PRINCIPAL DUTIES AND RESPONSIBILITIES:
1. Under general supervision, will breed and maintain common use mouse strains and use molecular (PCR) and cellular (flow cytometry) techniques to genotype transgenic and knockout mice.
2. Maintain records of mouse breeding using the Transnetyx Colony Management system.
3. Perform basic cell culture and in vitro assays and analyze by multi-parameter flow cytometry.
4. Perform DNA quantification, plasmid DNA isolation, and DNA analysis by restriction digest.
5. Perform in vivo experiments including tumor implantation, measurement, and treatment of tumor-bearing mice, as well as induction and monitoring of autoimmune disease.
6. Perform gene expression analyses using quantitative PCR.
7. Perform general lab management duties for both the Anderson Lab and the Transcriptomics Platform, including ordering supplies, maintaining reagent inventory, and assisting with the orientation of new staff.
8. Coordinate daily schedule of experiments.
9. Responsible for experimental data management: collect, record, tabulate, and present data. Create graphs of experimental data, perform statistical tests, and discuss progress of work regularly with supervisor.
10. Coordinate with medical staff to collect patient samples and process these samples to obtain single cell suspensions for downstream NGS library preparation.
11. Work with the Transcriptomics Platform manager to prepare both bulk and single cell RNA sequencing libraries
12. All other duties as assigned.
QUALIFICATIONS:
BS required.
New graduate with relevant course or project work.
SKILLS/ABILITIES/COMPETENCIES REQUIRED:
* High degree of computer literacy
* Experience working with mice is desired
* Sound analytical and organizational skills.
* Requires good oral and written communication skills.
* Must be able to logically and effectively structure tasks and set priorities
* Ability to identify potential problems and trouble-shoot solutions.
* Strong interpersonal communication skills.
WORKING CONDITIONS:
Normal laboratory environment.
Radioactive and hazardous materials are used in some of the experiments conducted.
Animal and human tissue will be used.
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