Biologist
4 weeks ago
This position will have several responsibilities in supporting CHO biologics pipeline, including genetic characterization of cell lines and cell banks. A strong candidate will have in-depth experience with a variety of molecular biology techniques and mammalian cell culture/aseptic technique, as well as exceptional attention to detail, demonstrated skills and productivity in laboratory research, and strong writing and communication skills.
Key Responsibilities:
· Characterize CHO cell lines using molecular biology techniques, including qPCR, ddPCR, Southern blot, and northern blot
· Sanger sequencing analysis
· Design of primer/probe reagents for gene copy number analysis
· Use of gel and blot imaging instruments
· Maintain detailed, accurate, and clear documentation of experiments and activities
· Effectively organize and communicate research progress and planned activities through oral and written presentations
· Collaborate with colleagues in the Cell Line Development group and across multiple functional areas of the Bioprocess Development and Launch department to advance the company Biologics Pipeline
· Maintain a high level of productivity in the lab
· Demonstrate scientific initiative and creative thinking to achieve project goals and contribute team performance in a climate of scientific rigor
· Contribute to the smooth and effective operation of the CHO Cell Line Development labs through the 5S program
· Understand and adhere to corporate standards regarding code of conduct, safety, appropriate handling of materials, and GxP compliance
Qualifications:
· Bachelor’s Degree or Master’s Degree in Cell/Molecular Biology or a related field with 5 years of relevant experience; pharmaceutical industry experience is preferred
· Expertise in molecular biology techniques, including DNA/RNA extraction and quantification, PCR-based assays, gel electrophoresis, blotting techniques and sequencing analysis
· Hands-on experience with mammalian cell culture
· Experience with DNA sequence manipulation software and common software packages such as Microsoft Office
· Excellent written and interpersonal communication skills with the ability to build strong relationships with peers and cross-functional team members
· Strong planning, multitasking, time management, and troubleshooting skills
· Collaborative and proactive attitude with strong scientific curiosity, work ethic, and attention to detail
· Authorization to work in the United States is required
Top Skills:
•Experience with expression vector elements, construction, and molecular cloning
•Experience with digital PCR assays, such as droplet digital PCR (ddPCR)
•Proven track record in vector biology, cell line genetic characterization, and/or CHO cell line development
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