Laboratory Technician

2 weeks ago

Bethesda, Maryland, United States GAP Solutions, Inc. Full time

Position Objective: Provide services as a Laboratory Technician in support of the overall functions of National Institute on Aging (NIA) within the National Institutes of Health (NIH). Under this task order, the contractor will independently provide support services to satisfy the overall operational objectives of the National Institute on Aging – Center for Alzheimer's and Related Dementias (CARD). The primary objective is to provide services and deliverables through performance of support services.

Duties and Responsibilities:

  • Work with staff on performing various experiments and procedures related to differentiation and phenotyping of induced pluripotent stem cell lines.
  • Work with staff to provide insight into the effects of ADRD-associated mutations on molecular readouts in cell culture.
  • Work with staff to characterize genetic pathways for Alzheimer's disease and related dementia (ADRD) using human cell-based models.
  • Work with staff to apply published protocols for cell differentiation to IPSC lines, microscopy, and molecular analysis. Program, operate, and implement novel methods on liquid handling platforms (Hamilton, Tecan).
  • Work with staff on performing large-scale iPSC cell culture experiments on liquid handling platforms. Support other research projects.
  • Collaborate with other team members to carry out large-scale cell culture protocols using high throughout robotic systems.
  • Perform standard immunological techniques, including western blotting and immunostaining.
  • Maintain laboratory equipment.
  • Operate laboratory equipment to analyze samples.
  • Analyze laboratory findings.
  • Train staff on the proper usage of laboratory instruments and equipment.
  • Provide assistance to laboratory staff in designing experiments, using instrumentation, data acquisition and analysis, and preparing figures for publication.
  • Work as a team to provide upkeep of scientific equipment and resources including reagents, stocks of frozen sells, animal samples including acquisition, documentation, and storage.
  • Program and use computers to store, process, and analyze data.
  • Monitor operational procedures in technical environments to ensure conformance to standards Interpret research or operational data.
  • Record research or operational data.
  • Develop new automated differentiation protocols for iPSC lines to differentiate into disease-relevant cell types like neurons, astrocytes, and microglia.
  • Develop image analysis routines to analyze the effects of disease-associated mutations on cellular phenotypes.
  • Clean Equipment - Daily
  • Meet with lab members to present updates - Weekly
  • Work products and documents related to characterizing genetic pathways for Alzheimer's disease and related dementia (ADRD) using human cell-based models; use IPSC lines to develop the differentiation protocols. - Weekly
  • Work products and documents related to evaluating the stability of differentiated cells across clines and passages to identify cell lines with desirable characteristics for subsequent cell engineering. - Weekly
  • Work products and documentation to develop differentiation, microscopy, and automated cell culture protocols. Work products and documentation resulting from large-scale automated cell culture phenotyping of iPSC lines. - Weekly
  • Work products and documents related to providing insight into the effects of ADRD-associated mutations on molecular readouts in cell culture; conduct independent research relating to the genetics of ADRD. - Weekly
  • Inspect Equipment - Weekly
  • Work products and documents related to applying published protocols for cell differentiation to IPSC lines, microscopy and molecular analysis. Work products and documents related to updating and maintaining laboratory notebooks and databases; collect, analyze and interpret data; prepare summaries and reports based on results and findings; draft and revise manuscripts for publication; attend meetings and present results and findings for staff discussion. - Bi-Weekly

Basic Qualifications:

  • Master's degree in cell biology, neurobiology, or a related discipline.
  • Minimum of one (1) year of related experience.
  • Skilled in Microsoft PowerPoint, Excel, and Word.
  • Experience with human iPSC culture and automated platforms.
  • Experience in scripting/coding to create, test, and perform new methods in automated cell culture platforms.

Minimum Qualifications:

  • Knowledge of basic Venus-based method scripting for Hamilton robot preferred.
  • Knowledge of proper laboratory disposal methods and procedures.
  • Team player in large research institutes.
  • Ability to multi-task and pay close attention to detail.
  • Excellent analytical, organizational and time management skills.
  • Strong communication skills, both oral and written.

  • Laboratory Technician

    2 weeks ago

    Bethesda, Maryland, United States Guidehouse Full time

    Job Family:Laboratory Technician (Digital)Travel Required:NoneClearance Required:Ability to Obtain Public TrustWhat You Will Do:We are currently searching for a Laboratory Technician. The primary objective is to perform a variety of laboratory techniques and procedures. This is a full-time onsite position located in Bethesda, MD. This requisition has been...

  • Laboratory Technician

    4 weeks ago

    Bethesda, Maryland, United States 22nd Century Technologies, Inc. Full time

    Job Title: Laboratory TechnicianLocation: Bethesda MD 20892Duration: 3 Years to Permanent Shift: 40 Hours/Week (Mon to Fri) Onsite: Yes Job Description:Laboratory Technician for Viral Immunology and Intravital Imaging Section (VIIIS) to perform a variety of laboratory techniques and procedures, especially managing mice colonies. Min Education: Master's...

  • Laboratory Technician

    1 month ago

    Bethesda, Maryland, United States 22nd Century Technologies, Inc. Full time

    Job Title: Laboratory TechnicianLocation: Bethesda MD 20892Duration: 3 Years to Permanent Shift: 40 Hours/Week (Mon to Fri) Onsite: Yes Job Description:Laboratory Technician for Viral Immunology and Intravital Imaging Section (VIIIS) to perform a variety of laboratory techniques and procedures, especially managing mice colonies. Min Education: Master's...

  • Bacteriology Technician

    4 weeks ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Bacteriology Technician

    1 month ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Animal Care Technician II

    1 month ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Animal Care Technician II

    4 weeks ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Cagewash Technician 1

    4 weeks ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Cagewash Technician 1

    1 month ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Animal Health Surveillance Lead/Technician

    2 weeks ago

    Bethesda, Maryland, United States Charles River Laboratories Full time

    Animal Health Surveillance Lead/Technician Req ID #: Location: Bethesda, MD, US, 20892 For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your...

  • Animal Care Technician I

    4 weeks ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Animal Care Technician I

    1 month ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Animal Health Surveillance Lead/Technician

    4 weeks ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Animal Health Surveillance Lead/Technician

    1 month ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Animal Health Surveillance Lead/Technician

    2 weeks ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Electrical Technician

    2 weeks ago

    Bethesda, Maryland, United States Delphinus Engineering Full time

    EOE Statement We are an equal employment opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status or any other characteristic protected by law. DescriptionGENERAL SUMMARY: Candidate will support Solid Waste/Thermal...

  • Cagewash Supervisor

    4 weeks ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Patient Care Coordinator

    2 weeks ago

    Bethesda, Maryland, United States ACI Federal Defunct Full time

    ACI Federal is seeking a Laboratory Technician to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute of Neurological Disorders and Stroke (NINDS) located in Bethesda, MD.Overall Position Summary and Objectives The purpose of this position is to support the lab day-to-day activities including coordination of lab...

  • Cagewash Attendant I

    4 weeks ago

    Bethesda, Maryland, United States Charles River Laboratories, Inc. Full time

    For 75 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will...

  • Research Support Specialist 1

    2 weeks ago

    Bethesda, Maryland, United States Ichgcp Full time

    Research Support Specialist 1 (Poolesville)Charles River Laboratories International Inc (CRL)Bethesda, MD, US, 20892*Research Support Specialist 1 (Poolesville)Req ID #:Location:Bethesda, MD, US, 20892 At Charles River, we are passionate about improving the quality of peoples lives. When you join our global family, you will help create healthier lives for...