Research Scientist III

4 weeks ago

Reston, Virginia, United States Coriell Institute for Medical Research Full time
Job Title: Research Scientist III

Coriell Institute for Medical Research is seeking a highly skilled Research Scientist III to join our Research and Development team. This role will play a critical leadership role in advancing our mission to innovate in stem cell research, genome editing, and biobanking.

Key Responsibilities:
  • Lead and support research technicians in laboratory activities, mentoring team members, and ensuring adherence to safety and quality protocols.
  • Reprogram primary human cell lines into induced pluripotent stem cells (iPSCs), ensuring high-quality standards.
  • Perform routine maintenance, expansion, and cryopreservation of human cell lines, documenting cell culture processes and ensuring traceability.
  • Characterize iPSC and differentiated cell lines using techniques such as immunohistochemistry, flow cytometry, and qPCR, ensuring consistency and reproducibility in experimental data.
  • Plan, execute, and analyze flow cytometry experiments to characterize cell surface markers and evaluate the quality and purity of iPSCs and differentiated cell types.
  • Execute precise CRISPR-Cas9 protocols for genome modification, contributing to the creation of isogenic cell lines.
  • Maintain detailed, accurate, and up-to-date laboratory records, ensuring all data are traceable and can be used in future projects and publications.
  • Oversee the maintenance and organization of lab inventories, reagents, media, and various instruments, ensuring smooth laboratory operations.
  • Collaborate with other members of the R&D team and other departments within the institute to share insights and develop best practices in cell biology and molecular genetics.
  • Stay up to date with advancements in stem cell technologies, genome editing, flow cytometry techniques, and related fields to enhance contributions to the R&D team.
Qualifications:
  • Master's or Ph.D. degree in Cell/Molecular Biology, Biotechnology, or a related field.
  • Minimum of 5 years of hands-on experience with iPSCs and human cell lines.
  • Proficiency in aseptic cell culture techniques and ability to troubleshoot and optimize protocols.
  • Expertise in molecular and cell biology techniques, including ELISA, qPCR, DNA/RNA isolation, immunohistochemistry, and flow cytometry.
  • Strong working knowledge of CRISPR-Cas9 genome editing with proven ability to validate and troubleshoot experiments.
  • Experience or familiarity with biobanking practices and cellular quality control standards.
  • Proven leadership and laboratory management experience, with the ability to mentor and guide research technicians.
  • Exceptional attention to detail, analytical skills, and ability to manage multiple projects in a dynamic research environment.
  • Meticulous recordkeeping and organizational skills, with a commitment to maintaining laboratory and biobank best practices.
  • Excellent communication skills and ability to work collaboratively within interdisciplinary teams.

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