RA II/Sr. RA Downstream Production

3 weeks ago

cambridge, United States Planet Pharma Full time

615942


Our client, a leading Biotechnology company in Cambridge. MA, is looking for a Research Associate II/Sr. RA or Downstream Production & Process Development to work at the bench to refold, purify, modify and QC TCR bispecifics and MHC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, Octet and analytical methods such as HPLC.


Required Skills:

  • BS/MS in Biochemistry, Biology, Bioengineering, Immunology, or a similar field.
  • 2+ years of academic or biotech/pharma laboratory experience.
  • Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is desirable.
  • Experience with protein refolding from inclusion bodies and/or protein modification is a plus.


Responsibilities:

  • Optimize protein refolding conditions from inclusion bodies.
  • Develop robust processes for protein purification from mammalian expression supernatant or from refolding, using Immobilized metal affinity, ion-exchange, and size exclusion chromatography.
  • Execute enzymatic and chemical protein modification protocols.
  • Generate purified and modified proteins at the 5-50 mg scale and maintain inventory.
  • Characterize recombinant proteins using SDS-PAGE, Western blot, protein quantitation, HPLC, ELISA, Octet and other biochemical/biophysical assays to determine protein purity and function.
  • Capture primary data, establish and maintain SOPs, and document work as required in Electronic Laboratory Notebook (ELN), maintaining up to date records.
  • Present data routinely to the Protein Sciences group and other multidisciplinary groups.


***Compensation within this range will be commensurate with level of experience***

  • RA II/Sr. RA Downstream Production

    3 weeks ago

    Cambridge, United States Planet Pharma Full time

    615942Our client, a leading Biotechnology company in Cambridge. MA, is looking for a Research Associate II/Sr. RA or Downstream Production & Process Development to work at the bench to refold, purify, modify and QC TCR bispecifics and MHC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, Octet and analytical methods such as HPLC.Required...

  • RA II/Sr. RA Downstream Production

    2 weeks ago

    Cambridge, United States Planet Pharma Full time

    615942Our client, a leading Biotechnology company in Cambridge. MA, is looking for a Research Associate II/Sr. RA or Downstream Production & Process Development to work at the bench to refold, purify, modify and QC TCR bispecifics and MHC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, Octet and analytical methods such as HPLC.Required...

  • RA II/Sr. RA Downstream Production

    3 weeks ago

    cambridge, United States Planet Pharma Full time

    615942Our client, a leading Biotechnology company in Cambridge. MA, is looking for a Research Associate II/Sr. RA or Downstream Production & Process Development to work at the bench to refold, purify, modify and QC TCR bispecifics and MHC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, Octet and analytical methods such as HPLC.Required...

  • RA II/Sr. RA Downstream Production

    2 weeks ago

    Cambridge, MA, United States Planet Pharma Full time

    615942Our client, a leading Biotechnology company in Cambridge. MA, is looking for a Research Associate II/Sr. RA or Downstream Production & Process Development to work at the bench to refold, purify, modify and QC TCR bispecifics and MHC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, Octet and analytical methods such as HPLC.Required...

  • Senior Researcher

    3 weeks ago

    Cambridge, United States Planet Pharma Full time

    Role OverviewWe are seeking a passionate Research Associate II/Senior RA to support downstream production and process development of TCR bispecifics and MHC proteins on our Protein Engineering and Molecular Sciences team. The ideal candidate will have hands-on experience in protein purification, with preferred experience refolding proteins from inclusion...

  • Senior Researcher

    3 weeks ago

    cambridge, United States Planet Pharma Full time

    Role OverviewWe are seeking a passionate Research Associate II/Senior RA to support downstream production and process development of TCR bispecifics and MHC proteins on our Protein Engineering and Molecular Sciences team. The ideal candidate will have hands-on experience in protein purification, with preferred experience refolding proteins from inclusion...

  • Senior Researcher

    3 weeks ago

    cambridge, United States Planet Pharma Full time

    Role OverviewWe are seeking a passionate Research Associate II/Senior RA to support downstream production and process development of TCR bispecifics and MHC proteins on our Protein Engineering and Molecular Sciences team. The ideal candidate will have hands-on experience in protein purification, with preferred experience refolding proteins from inclusion...

  • Director - GRA CMC, Small Molecules

    3 weeks ago

    cambridge, United States Takeda Full time

    Job DescriptionAt Takeda, we are a forward-looking, world-class R&D organization that unlocks innovation and delivers transformative therapies to patients. By focusing R&D efforts on four therapeutic areas and other targeted investments, we push the boundaries of what is possible in order to bring life-changing therapies to patients worldwide.Join Takeda as...

  • Director - GRA CMC, Small Molecules

    3 weeks ago

    Cambridge, United States Takeda Full time

    Job DescriptionAt Takeda, we are a forward-looking, world-class R&D organization that unlocks innovation and delivers transformative therapies to patients. By focusing R&D efforts on four therapeutic areas and other targeted investments, we push the boundaries of what is possible in order to bring life-changing therapies to patients worldwide.Join Takeda as...

  • Director - GRA CMC, Small Molecules

    3 weeks ago

    cambridge, United States Takeda Full time

    Job DescriptionAt Takeda, we are a forward-looking, world-class R&D organization that unlocks innovation and delivers transformative therapies to patients. By focusing R&D efforts on four therapeutic areas and other targeted investments, we push the boundaries of what is possible in order to bring life-changing therapies to patients worldwide.Join Takeda as...

  • Director - GRA CMC, Small Molecules

    2 weeks ago

    Cambridge, United States Takeda Full time

    Job DescriptionAt Takeda, we are a forward-looking, world-class R&D organization that unlocks innovation and delivers transformative therapies to patients. By focusing R&D efforts on four therapeutic areas and other targeted investments, we push the boundaries of what is possible in order to bring life-changing therapies to patients worldwide.Join Takeda as...

  • Regulatory Operations Director

    4 weeks ago

    Cambridge, Massachusetts, United States Ipsen Pharma Full time

    Job Title: Regulatory Operations DirectorAt Ipsen Pharma, we are seeking a highly skilled Regulatory Operations Director to join our team. This is a key leadership role that will be responsible for formulating and implementing operational management creating a best-in-class US regulatory operations team and technology leadership/ownership of Ipsen's...

  • Sr. Research Associate

    5 days ago

    Cambridge, United States Planet Pharma Full time

    Cambridge, MAFull time, permanent role100% onsite Research Associate II/Senior Research Associate, Quality Control & Analytical DevelopmentNotes:Looking for 2 plus years CDMO experienceWill be stress testing protein assays for eventual tech transfer to CDMO.Must have experience characterizing proteins/antibodies and developing assays using techniques such as...

  • Sr. Research Associate

    5 days ago

    Cambridge, United States Planet Pharma Full time

    Cambridge, MAFull time, permanent role100% onsite Research Associate II/Senior Research Associate, Quality Control & Analytical DevelopmentNotes:Looking for 2 plus years CDMO experienceWill be stress testing protein assays for eventual tech transfer to CDMO.Must have experience characterizing proteins/antibodies and developing assays using techniques such as...

  • Sr. Manager, Regulatory Strategy

    6 months ago

    Cambridge, United States Moderna, Inc. Full time

    The Role:Moderna Therapeutics is seeking a Sr. Manager of Regulatory Strategy based in Cambridge headquarters. This is a newly created role intended for a forward looking, creative and agile regulatory manager able to anticipate and address the challenges involved in gaining market approval for messenger RNA therapeutics as unprecedented new drug modality....

  • Planet Pharma | Sr. Research Associate | cambridge, ma

    4 days ago

    cambridge, United States Planet Pharma Full time

    Cambridge, MAFull time, permanent role100% onsite Research Associate II/Senior Research Associate, Quality Control & Analytical DevelopmentNotes:Looking for 2 plus years CDMO experienceWill be stress testing protein assays for eventual tech transfer to CDMO.Must have experience characterizing proteins/antibodies and developing assays using techniques such as...

  • Planet Pharma | Sr. Research Associate | cambridge, ma

    5 days ago

    cambridge, United States Planet Pharma Full time

    Cambridge, MAFull time, permanent role100% onsite Research Associate II/Senior Research Associate, Quality Control & Analytical DevelopmentNotes:Looking for 2 plus years CDMO experienceWill be stress testing protein assays for eventual tech transfer to CDMO.Must have experience characterizing proteins/antibodies and developing assays using techniques such as...

  • Director, Research Finance and Operations

    5 months ago

    Cambridge, United States Joslin Diabetes Center Full time

    The Director of Research Finance & Operations (Fiscal Services) provides leadership and supervision of Grants financial management of Research Administration and ensures compliance with federal/state federal laws and other regulations/requirements. The Director will maintain the Centers financial/information systems to support the Centers research activities...

  • Director of Regulatory Affairs and Quality Assurance

    3 weeks ago

    Cambridge, United States Crescent City Recruitment Group Full time

    Director of Regulatory Affairs & Quality Assurance (RA/QA) We're working closely with the C-suite at an exciting medical-device company to recruit a Director of Regulatory Affairs & Quality Assurance, who will lead the development and execution of comprehensive regulatory and quality systems. This role is pivotal in both securing immediate FDA 510(k)...

  • Senior Director and Regulatory Platform Lead GRA CMC, Device and Drug-Device Combinations Material Sciences Expert

    4 weeks ago

    Cambridge, Massachusetts, United States Takeda Pharmaceutical Full time

    Job DescriptionAt Takeda, we are a forward-looking, world-class R&D organization that unlocks innovation and delivers transformative therapies to patients. By focusing R&D efforts on three therapeutic areas and other targeted investments, we push the boundaries of what is possible to bring life-changing therapies to patients worldwide.Key...