RA II/Sr. RA Downstream Production

2 weeks ago


Cambridge MA United States Planet Pharma Full time

615942


Our client, a leading Biotechnology company in Cambridge. MA, is looking for a Research Associate II/Sr. RA or Downstream Production & Process Development to work at the bench to refold, purify, modify and QC TCR bispecifics and MHC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, Octet and analytical methods such as HPLC.


Required Skills:

  • BS/MS in Biochemistry, Biology, Bioengineering, Immunology, or a similar field.
  • 2+ years of academic or biotech/pharma laboratory experience.
  • Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is desirable.
  • Experience with protein refolding from inclusion bodies and/or protein modification is a plus.


Responsibilities:

  • Optimize protein refolding conditions from inclusion bodies.
  • Develop robust processes for protein purification from mammalian expression supernatant or from refolding, using Immobilized metal affinity, ion-exchange, and size exclusion chromatography.
  • Execute enzymatic and chemical protein modification protocols.
  • Generate purified and modified proteins at the 5-50 mg scale and maintain inventory.
  • Characterize recombinant proteins using SDS-PAGE, Western blot, protein quantitation, HPLC, ELISA, Octet and other biochemical/biophysical assays to determine protein purity and function.
  • Capture primary data, establish and maintain SOPs, and document work as required in Electronic Laboratory Notebook (ELN), maintaining up to date records.
  • Present data routinely to the Protein Sciences group and other multidisciplinary groups.


***Compensation within this range will be commensurate with level of experience***



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