Biologist
3 weeks ago
One year contract position with benefits.
Roles/Responsibilities:
Perform research on neurodevelopment and neuroprotection using primary cells in culture and in vivo mouse models.
Design and perform experiments: maintain stem cells, primary neurons and plan all details to understand the role of GPCR ligands in:
- Neurogenesis/stem cell differentiation.
- Neurite growth, synaptogenesis
- Signaling/gene expression
- Recovery in mouse models of spinal cord injury
Analyze the data, summarize and prepare reports for presentation and/or publication
Sharing technical knowledge with staff and trainees on biochemical and cell biological experiments: including reporter assays, cell culture, biochemical, immunocytochemical and molecular biological, and confocal microscopic imaging procedures.
General laboratory responsibilities:
- Prepare purchase request forms for laboratory supplies.
- Assist junior lab members in all basic laboratory procedures and safe procedures.
- Provide support for the use of common laboratory equipment.
- Perform genotyping of mouse lines.
- Maintain the mouse lines.
- Build experimental setups necessary for the on-going research.
Minimum Qualifications:
MS or Ph.D. in biochemistry, cell biology, neuroscience
At least 5 years of experience in maintaining and primary neural cell culture and differentiation into neurons
Experience in mouse surgery including spinal cord injury
At least 5 years of experience in immunocytochemistry and confocal microscopy (experience with Zeiss microscopy is preferred)
Demonstrated expertise in cell-based in vitro assays; qPCR, western blotting, cAMP assay, calcium assay, synaptogenesis
Experience in FACS analysis
Able to independently analyze data, troubleshoot, and solve problem
Excellent record keeping, ability to follow precise steps in a protocol
Experience with Microsoft office like Excel, Graph Pad prism for data analysis is preferred
Ability to work independently
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