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Research Assistant
4 months ago
The Laboratory of Structural Biology and Biophysics, led by Steve Bonilla, is dedicated to unraveling the fundamental physical principles that govern the three-dimensional shape and dynamic behavior of RNA molecules. Specifically, our focus lies in understanding the RNA sequences encoded by viruses, which enable them to manipulate the biochemical machinery of their host. To achieve this goal, we employ a wide range of cutting-edge tools and techniques, including cryogenic electron microscopy (cryo-EM), single-molecule FRET, small-angle X-ray scattering (SAXS), as well as other biophysical and structural biology methods, alongside functional assays.
Overview
The research assistant will support research conducted at the Laboratory of Structural Biology and Biophysics, assisting with molecular biology and structural characterization of RNA and RNA-protein complexes.
Responsibilities
Responsibilities include:
- Designing DNA templates and primers, PCR amplification, DNA purification
- Producing RNA by in vitro transcription and purify by gel electrophoresis
- Expressing and purifying proteins
- Analyzing, presenting, and discussing data
- Preparing samples for cryo-EM studies
- Preparing and maintaining buffers and reagents
Required:
- Must have previous laboratory training experience in a research lab
- Ability to follow protocols
- Proficiency using basic laboratory equipment such as centrifuges, spectrophotometers, and pipettes
- Ability to plan and perform molecular biology experiments including primer design, PCR, gel electrophoresis, DNA extraction, cloning, and sequencing
- Familiarity with protein purification
- Ability to prepare and maintain reagents and solutions (e.g., buffers)
- Ability to clearly convey scientific concepts to colleagues and supervisors
- Previous experience working with RNA is a plus
- Previous experience in a structural biology lab is a plus
- Experience with cryo-electron microscopy is a plus
- Experience expressing and purifying proteins
- Programming skills is a plus
- Having independently worked on their own research project is a plus
The salary of the finalist selected for this role will be set based on various factors, including but not limited to organizational budgets, qualifications, experience, education, licenses, specialty, and training. The hiring range provided represents The Rockefeller University's good faith and reasonable estimate of the range of possible compensation at the time of posting.
Compensation Range: Min
USD $48,300.00/Yr.
Compensation Range: Max
USD $52,000.00/Yr.
