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Research Associate
3 months ago
Lab/Area:Bruneau Lab
Description:
Research Associate – Bruneau Lab
Who we are:
The Gladstone Institutes is an independent, not-for-profit research institution. Its mission is to improve the health and well-being of all people through medical research and education in neuroscience, cardiovascular biology, immunology, and stem cell biology. Home to notable leaders in science and biomedicine, Gladstone comprises five institutes as well as various centers and programs. All of its approximately 600 members are aligned in their mission to carry out or support science that overcomes disease. Located in an award-winning building at the Mission Bay Campus, Gladstone offers a competitive salary and benefits program. It provides an ideal environment for those seeking to maximize and fully leverage their potential. Consider Gladstone and make a difference in human health.
About the role:
The laboratory of Benoit Bruneau at the Gladstone Institutes ) is searching for a Research Associate (RA) to help us study the regulation of genes during heart development, using mouse models in vivo and stem cells in vitro. We are looking for candidates who are strongly motivated, work well in a team, and who could start July-August 2024. There is a potential for one or two publications within a two-year time period. Candidates with plans for graduate school, medical school or dental school are encouraged to apply.
What you will do:
We are looking for a research associate whose primary role will focus on culturing induced pluripotent stem cell (iPSC) and performing molecular biology techniques to understand gene expression regulation during in vitro cardiac differentiation.
iPSC culture: maintain and expand iPSC lines, including routine passaging, freezing, and thawing of cells Differentiation protocols: use differentiation protocols to generate cardiomyocytes, ensuring consistency and reproducibility, and checking differentiation efficiency by flow cytometry Molecular biology techniques: perform molecular biology techniques such as PCR, RT-qPCR, and western blotting to analyze gene expression and protein levels Use sequencing techniques such as RNA-seq, ChIP-seq, and ATAC-seq to investigate gene regulation and epigenetic changes in iPSC-derived cardiomyocytes Analyze experimental and sequencing data, and interpret results Updates records and log books and assists in maintaining lab records and inventory Assist in lab management functions including ordering reagents, organizing laboratory inventory What you will need: Associate's degree and 2+ years of relevant experience BA/BS in Biological sciences and at least 6 months practical experience in a research laboratory (preferred) Excellent verbal and written communication skills Strong work ethic Strong organizational skills with attention to detail and the ability to multitask Excellent communication and interpersonal skills, with the ability to work effectively in a team setting. What is preferred: iPSC or cell culture Flow cytometry Molecular biology skills such as PCR, western blotting, RT-qPCR Lab management (ordering, restocking, database maintenance) What is nice to have: R or python programming (or equivalent) Experience with next-generation sequencing, eg RNA-seq, ChIP-seq CRISPR/Cas9 genome editing Proficiency in Excel, Word, ImageJ Fiji, Photoshop and Illustrator If this role is not for you: Please stay in touch and to see a complete list of our open career opportunities, please visit: Hiring Range: $25-$29 hrGladstone Perks & BenefitsPeople–work with talented, committed, and supportive teammates within an organization that values each member of its community.A meaningful place to grow and learn–whether it's your professional skills or scientific knowledge, we have the resources and environment to advance either so you can better support Gladstone's mission to drive a new era of discovery in disease-oriented science and to mentor tomorrow's leaders in an inspiring and diverse environment.Healthy work/life balance–you are highly engaged and productive at work because you can have time to recharge and enjoy a vibrant life outside of work.Compensation–competitive salary. Title and salary will be commensurate with education and experience.Excellent benefits–generous medical, dental, vision, retirement plan, paid vacation, commuter benefits, access to free shuttle transportation.
Gladstone is committed to providing equal employment opportunities to all employees and applicants for employment without regard to race, color, sex, religion, national origin, ancestry, age, marital status, medical condition, physical or mental disability, veteran status, sexual orientation, or any other non-job related characteristic. We make all employment decisions so as to further this principle of equal employment.
